Rabbit anti-RNA Polymerase II Antibody, Affinity Purified
Brand:
A304-405A-T
- Brand
- Bethyl Laboratories
- Application
- ChIP-PCR
IP
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 754 and 804
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- RNA Polymerase II
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, polymerase (RNA) II subunit A, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPBh1, RpIILS, RPO2, RPOL2
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- Not recommended. A304-405A has not performed satisfactorily when used for WB of RNA Polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA Polymerase II.
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- ChIP-PCR
- 8 µg per IP
- Target Sentence
- RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].
- Product Link
- http://www.bethyl.com/product/A304-405A
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