Rabbit anti-Sin1 Antibody, Affinity Purified
Brand:
A300-910A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- between 470 and C-term
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- Sin1
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- JC310, MEKK2-interacting protein 1, MIP1, mitogen-activated protein kinase 2-associated protein 1, mSIN1, ras inhibitor MGC2745, SAPK-interacting protein 1, SIN1, SIN1b, SIN1g, stress-activated map kinase interacting protein 1, Stress-activated map kinase-interacting protein 1, stress-activated protein kinase-interacting 1, target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Chicken, Sheep, Bovine and Orangutan
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:2,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 5 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- Stress-activated map kinase-interacting protein 1 (Sin1) is highly similar to the yeast stress- activated protein kinase SIN1. Sin1 is an essential mTORC2 subunit that is required to act as the PDK2 that phosphorylates Akt/PKB at Ser 473. Sin1 has been implicated as a scaffolding protein. Sin1 is required for rictor binding in the mTORC2 complex. Sin1 has also been shown to be important to the SAPK (stress-activated kinase) signaling pathway and in the nucleus may act as a scaffold between ATF-2 and p38 to facilitate p38-induced phosphorylation of ATF-2. Sin1 is also found to form a complex with c-Jun N-terminal kinase (JNK) and may similarly function as a scaffolding protein in JNK signaling.
- Product Link
- http://www.bethyl.com/product/A300-910A
Related products
