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Rabbit anti-U2AF65 Antibody, Affinity Purified

Catalog No.
A303-667A-M
Pack Size
  • 20 µl (2 blots)
  • 100 µl (10 blots)
  • 100 µl (1000 µg/ml)
Quantity
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Brand:
Specifications
A303-667A-M
Brand
Bethyl Laboratories
Application
IHC
IP
WB
Storage Conditions
2 - 8° C
Host
Rabbit
Conjugation
Unconjugated
Immunogen
Between 430 and 475
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Reactivity
Human
Target
U2AF65
Shelf Life
1 year from date of receipt
Antibody Type
Polyclonal
Alternate Names
hU2AF(65), hU2AF65, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit, U2AF65
Format
Whole IgG
Presumed Reactivity
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Product Type
Primary Antibodies
Product Sub Types
Primary Antibodies
UNSPSC
12352203
Western Blot (WB)
1:2,000 - 1:10,000
Immunoprecipitation (IP)
2 - 10 µg/mg lysate
Immunohistochemistry (IHC)
1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Sentence
U2 auxiliary factor (U2AF) is comprised of a large and a small subunit and is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. Splicing factor U2 auxiliary factor 65 kDa subunit (U2AF65) is the large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. U2AF65 binds to the polypyrimidine tract of introns early during spliceosome assembly [taken from NCBI Entrez Gene (Gene ID: 11338)].
Product Link
http://www.bethyl.com/product/A303-667A
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