A303-666A-M
- Brand
- Bethyl Laboratories
- Application
- IHC
IP
WB
- Storage Conditions
- 2 - 8° C
- Host
- Rabbit
- Conjugation
- Unconjugated
- Immunogen
- Between 380 and 430
- Buffer
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
- Reactivity
- Human
- Target
- U2AF65
- Shelf Life
- 1 year from date of receipt
- Antibody Type
- Polyclonal
- Alternate Names
- hU2AF(65), hU2AF65, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit, U2AF65
- Format
- Whole IgG
- Presumed Reactivity
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
- Product Type
- Primary Antibodies
- Product Sub Types
- Primary Antibodies
- UNSPSC
- 12352203
- Western Blot (WB)
- 1:2,000 - 1:10,000
- Immunoprecipitation (IP)
- 2 - 10 µg/mg lysate
- Immunohistochemistry (IHC)
- 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Target Sentence
- U2 auxiliary factor (U2AF) is comprised of a large and a small subunit and is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. Splicing factor U2 auxiliary factor 65 kDa subunit (U2AF65) is the large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. U2AF65 binds to the polypyrimidine tract of introns early during spliceosome assembly [taken from NCBI Entrez Gene (Gene ID: 11338)].
- Product Link
- http://www.bethyl.com/product/A303-666A