XR-9740
- Brand
- ProSci
- Specificity
- Minimum Cross reactivity to Human Serum Proteins
- Host
- Goat
- Isotype
- IgG
- Immunogen
- Mouse IgG - whole molecule
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
- Application Note
- Biotin Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).
- Product Link
- https://www.prosci-inc.com/product/goat-anti-mouse-igg-hl-antibody-biotin-9740/
- Storage
- Biotin Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 ˚C prior to restoration. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 ˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use.
- Tested Applications
- E
- Conjugate
- Biotin
- Physical State
- Lyophilized
- Species Reactivity
- Mouse
- By Species
- Anti-Mouse
- By Conjugate
- Biotin
- Secondary Antibody Target Species
- Reacts with the heavy chain of mouse IgG.