Co-capping T7 in vitro transcription reagent (pUTP, CAP GAG (3'Ome))

Co-capping T7 in vitro transcription reagent is a co-transcription capping reagent for in vitro transcription of 5'capped mRNA to produce Cap-1 structure with an optimized RNA synthesis formulation. The kit is capable of synthesizing mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield using linear double-stranded DNA containing T7 promoter followed by an AG initiation sequence. The kit contains modified nucleotides (pUTP) as alternative. Capped mRNAs with modified nucleotide have lower immunogenic potential. Each standard reaction yields up to 150-200 μg of RNA from 1μg DNA template. Reaction size can be scaled up to yield milligram-level RNA as needed. RNA synthesized from the kit is suitable for many applications including RNA structure and function studies, transfections, microinjections, in vitro translation, preclinical mRNA therapeutic mRNA studies.