exTEN II PCR Master Mix (2X)

exTEN II is an unique premixed blend of Taq DNA polymerase, a proof-reading enzyme, dNTPs, MgCl2 and reaction buffer for amplification of DNA templates by PCR With the addition of a 3’ 5’ exonuclease (proof-reading) ability, the amplification efficiency is enhanced through a lower rate misincorporated nucleotides compared to just Taq DNA Polymerase alone.

exTEN II PCR Master Mix produces higher yields and amplifies longer fragments up to 10kb. Most of the amplified DNA fragments have 3’A overhang, while a small percentage are blunt-end. This premix formulation saves times and reduces contamination by reducing the number of pipetting steps for PCR setup. exTEN II PCR Master Mix consists of a density reagent and 2 tracking dyes which migrates at the same rate as a 4000bp and 50bp DNA fragment in a 1% agarose gel.